Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVQII-FDSKTGNVQRFVNKTGFQQIR-KVDEMDH--VDTPFVLVTYTTN----FGQVPASTQSFL--EKYAHLLLGVAASGNKVWGDNFAKSADTISRQYQVPILHKFELSGTSKDVELFTQEVERVVTKSSAKMDPVK 3N3B Chain:C ((23-139)) --QLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYILIVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIE---------------------

General information: TITO was launched using: RESULT: Template: 3N3B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 474 -60012 -126.61 -560.86 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -126.61 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.400

(partial model without unconserved sides chains): PDB file : Tito_3N3B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N3B-query.scw PDB file : Tito_Scwrl_3N3B.pdb: