Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHITTKRLLIREFEFKDWQAVYEYTSDSNVMKY-IPEGVFTEEDAKAFVNKNKGDNAEKFPVILRDEDCLIGHIVFYKY-FGEHTYEIGWVFNPNYQNKGYASEAAQAILEYGFKEMNLHRIIATCQPENIPSYRVMKKIGMRREGFFKKCIPKGNEWWDEYYYAILEEEWN 4R87 Chain:A ((5-146)) ----NSQLTLRALERGDLRFIHNLNNNRNIMSYWFEEPYESFDELEELYNKHIHDNAERRFVVEDAQKNLIGLVELIEINYIHRSAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEG--------------------------

General information: TITO was launched using: RESULT: Template: 4R87.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 513 -82632 -161.08 -590.23 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -161.08 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.618

(partial model without unconserved sides chains): PDB file : Tito_4R87.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R87-query.scw PDB file : Tito_Scwrl_4R87.pdb: