Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLCREVWIEVNLDAVKKNLRAIRRHIPHKSKIMAVVKANGYGHGSIEVARHALEHGASELAVASVEEGIVLRKAGITAPILVLGFTSLSCVKKSAAWNITLSAFQVDWMKEANEILEKEASANRLAIHINVDTGMGRLGVRTKEELLEVVKALKASKFLRWTGIFTHFSTADEPDTTLTKLQHEKFISFLSFLKKQGIELPTVHMCNTAAAIAFPEFSADMIRLGIGLYGLYPSAYIKQLNLVKLEPALSLKARIAYVKTMRTEPRT-VSYGATYIAEPNEVIATLPIGYADGYSRALSNRGFVLHRGKRVPVAGRVTMDMIMVSLGENGEGKQGDEVVIYGKQKGAEISVDEVAEMLNTINYEVVSTLSRRIPRFYIRDGEIFKVSTPVLYV 4LUS Chain:B ((3-384)) --KITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYGHGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKITMTVYSLETAQKINEI--AKSLGEKACVHVKIDS-----GFQPNEESVQEIIELNKLEYIDLEGMFTHFATADE---EYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLRLNMVRAGIILYGHYPSDDVFKDRL-ELRPAMKLKSKIGHIK--QVEPGVGISYGLKYTTTGKETIATVPIGYADGFTRIQKNPK-VLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEVILFGE---GEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLL--

General information: TITO was launched using: RESULT: Template: 4LUS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2098 -197602 -94.19 -529.76 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -94.19 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_4LUS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LUS-query.scw PDB file : Tito_Scwrl_4LUS.pdb: