Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFLLDLFTNWTFDKVLDYTLAAVIWSVFKSRSKQNKYPGYFEKIR---------RYRNLHPLLRSLSMRVLLSITIHPYMFS 2WL8 Chain:A ((195-227)) ---------------------------------ITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCK-----------------

General information: TITO was launched using: RESULT: Template: 2WL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 19 1136 59.76 47.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : 59.76 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.421

(partial model without unconserved sides chains): PDB file : Tito_2WL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WL8-query.scw PDB file : Tito_Scwrl_2WL8.pdb: