Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLKIDIKGRGKYKPASDYSIDDVKNVLMEKIFEESSRIINSDDDLEIIEKVDFRTDKINVLSLFSGCGGLDLGFELAGLAAVIGEQAAMEAFKDKDRFNELRNKSIFHTIYTNDLFKEANQTYKTNFPGHVIQHEKDIRQV--KYFPKCNLILGGFPCPGFSEAGP-RLIDDDRNFLYLHFIRSLIQAQPEIFVAENVKGMMTLGKGEVLNQIIEDFASAGYRVQFKLLNARDYGVPQLRERVIIEGVRKDISF-NYKYPSPTHGEETGLKPFKTLRDSIGDLVTDPGPYFTGSYS-------SIYMSRNRKKSWDEQSFTIQASGRQAPLHPGGLSMKKIGKDKWVFPDGEENHRRLSVKEIARVQTFPDWFQFSQGTNSQTSINNRLDKQYKQIGNAVPVLLAKAVASPIANWAINYLESSPNNKIKNRERKLSIRTFLRIKTS 8MHT Chain:A ((1-320)) -----------------------------------------------MIEIKDKQLTGLRFIDLFAGLGGFRLALESCGAECV----------------------------YSNEWDKYAQEVYEMNFGE---KPEGDITQVNEKTIPDHDILCAGFPCQAFSISGKQKGFEDSRGTLFFDIARIVREKKPKVVFMENVKNFASHDNGNTLEVVKNTMNELDYSFHAKVLNALDYGIPQKRERIYMICFRNDLNIQNFQFPKPFE-LNTFVKDLLLPDSEVEHLVIDRKDLVMTNQEIEQTTPKTVRLGIVGKGGQGERIYSTRGIAITLSAYGGGIFAKTGG----YLVNGKT--RKLHPRECARVMGYPDSYKVHPSTS----------QAYKQFGNSVVINVLQYIAYNIGS--------------------------------

General information: TITO was launched using: RESULT: Template: 8MHT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1521 21152 13.91 68.45 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 13.91 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_8MHT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-8MHT-query.scw PDB file : Tito_Scwrl_8MHT.pdb: