Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPTEIYVDGASAGNPGPSGIGIFIKHEGKAESFSIPIGVHTNQEAEFLALIEGMKLCATRGYQSVSFRTDSDIVERATELEMVKNITFQPF----------VEEIIRLKAAFPLFFIK--WIPGK----QNQKADLLAKEAIRLNEKN 1GOB Chain:A ((6-140)) ---EIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMELMAAI--VALEALKEHCEVILSTDSQYVRQAI-TQWIHNWKKRGWKTADKKPVKNVDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAA-------

General information: TITO was launched using: RESULT: Template: 1GOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 498 -30432 -61.11 -255.73 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -61.11 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1GOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GOB-query.scw PDB file : Tito_Scwrl_1GOB.pdb: