Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------------MTQLLIIFAAAAAGLFFFEDVRDVLKLWDIRDMRIIWYGVSIAVIVILADMAVMKWFPSHLYDDGGINKKIFSKRSIPHIIFLTLLIAFAEEMLFRGV----LQTHIGLWTASLIFAALHFRY--------------------LSKWLLFIMVTAISFLLGLMYEWTGN-LFVPMTAHFIIDAVFA---CQIRFEHVRRDKHDEHVESREKKSPESL--- 4CAD Chain:C ((8-269)) NPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIAKKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPF----GGSISQFQFSGGDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVNNSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAITQDTKIIETGVLFLVAAAIILYDKKMFFEK

General information: TITO was launched using: RESULT: Template: 4CAD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 477 -79475 -166.61 -429.59 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.61 3D Compatibility (PKB) : -166.61 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_4CAD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CAD-query.scw PDB file : Tito_Scwrl_4CAD.pdb: