Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKIGYRTIKTALGTALAIYISQLLHLQNFASAGIITILCIQITQKRSLQASWARFSACCLAIAFSYLFFELIGYHPFVIGALLLIFIPITVLLKINEGIVTSSVIILHLYMSGGITPTFIWNEVQLITVGIGVALLMNLYMPSLDRKLIAYRKKIEDNFAVIFAEIERYLLTGEQDWSGKEIPETHQLITEAKNLAYRDVQNHILRYENLHYHYFKMREKQFEIIERLLPKVTSISITVDQGKMIAEFIHDLREAIHPGNTAYKFLKRLADMRKEFEEMPLPATREEFEARAALFHLLGEMEQYLVIKSYFKGIKAQKSLG 2F6M Chain:B ((21-46)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TSKDKEVIETLSEIYSIVITLDHVEK---------------

General information: TITO was launched using: RESULT: Template: 2F6M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2 140 69.75 5.37 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain B : 0.46 3D Compatibility (PKB) : 69.75 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.46 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_2F6M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F6M-query.scw PDB file : Tito_Scwrl_2F6M.pdb: