Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKFEASIDNLKEIEMNAYAYELIREIVLPDMLGQ-----DYSSMMYWAGKHLARKFPLE------------SWEEFPAFFEEAGWGTL-----TNVSA---KKQELEFELEGPIISNRLKH----QKEPCFQLEAGFIAEQIQLMNDQIAESYEQVKKRADKVVLTVKWDMKDPV 2J3W Chain:B ((22-177)) --------------TEVSVSAFALLFSEMVQYCQSRVYSVSELQARLADMGQGVGASLLDVLVMREKNGKRETKVLNILLFIKVNVWKALFGKEADKLEQANDDDKTYYIIEKEPLINAYISVPKENSTLNCAAFTGGIVEAILT-HSGFPAKVTVHWH---KGTTLMIKFDES---

General information: TITO was launched using: RESULT: Template: 2J3W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 379 7149 18.86 56.29 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 18.86 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.299

(partial model without unconserved sides chains): PDB file : Tito_2J3W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J3W-query.scw PDB file : Tito_Scwrl_2J3W.pdb: