Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTISVNGEK--NTEVTDIPDHAVAVKMLLNKLTEFGIIKDLNE-IDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPLHNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLYSLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRSGNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETALEVFASRIHKYIGSYAARMSGVDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALN---NVRGEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK 1X3N Chain:A ((20-407)) ---VLVINCGSSSIKFSVLDVATCDVLMAGIADGMNTENAF--LSINGDKPINLAHSNYEDALKAIAFELEK-------RDLTDSVALIGHRIAHGGELFTQSVIITDEIIDNIRRVSPLAPLHNYANLSGIDAARHLFPAVRQVAVFDTSFHQTLAPEAYLYGLPWEYFSSLGVRRYGFHGTSHRYVSRRAYELLDLDEKDSGLIVAHLGNGASICAVRNGQSVDTSMGMTPLEGLMMGTRSGDVDFGAMAWIAKETGQTLSDLERVVNKESGLLGISGLSSDLRVLEKAWHEGHERARLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSVLIRQLVIEHLGVLGLTLDVEMNKQPNSHGERIISANP-SQVICAVIPTNEEKMIALDAIHLGN

General information: TITO was launched using: RESULT: Template: 1X3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2098 -48804 -23.26 -127.76 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -23.26 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_1X3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X3N-query.scw PDB file : Tito_Scwrl_1X3N.pdb: