Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKLLTMAMPK-GRIFEEAAGLLRQAGYRLPEEFEDSRKLIIDVPEENLRFILAKPMDVTTYVEHGVADVGIAGKDVMLEE---------ERDVYEVLDLNISKCHLAVAGLPNTDWSGV----APRIATKYPNVASSYFREQGEQVEIIKLNGSIELAPLIGLADRIVDIVSTGQTLKENGLVETEHICDITSRFIVNPVSY-RMKDDVIDEMASRLSLVVEGETAK 1H3D Chain:A ((7-234)) ----LRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAENMPIDILRV---RDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFGGCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPRAGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQGVIQARESK

General information: TITO was launched using: RESULT: Template: 1H3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1002 39544 39.47 191.96 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : 39.47 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_1H3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H3D-query.scw PDB file : Tito_Scwrl_1H3D.pdb: