Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQSPIFLTPVFKEKIWGGTALRDRFGYSIPSESTGECWAISAHPKGPSTVANGPYKGKTLIELWEEHREVFGGVEGDRFPLLTKLLDVKEDTSIKVHPDDYYAGENEEGELGKTECWYIIDCKENAEIIYGHTARSKTELVTMINSGDWEGLLRRIKIKPGDFYYVPSGTLHALCKGALVLETQQNSDATYRVYDYDRLDSNGSPRELHFAKAVNAATVPHVDGYIDESTESRKGITIKTFVQGEYFSVYKWDINGEAEMAQDESFLICSVIEGSGLLKYEDKTCPLKKGDHFILPAQMPDFTIKGTCTLIVSHI 4FAH Chain:A ((302-334)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VFQVFEGAGAVVMNGETTKLEKGDMFVVPSWVP---------------

General information: TITO was launched using: RESULT: Template: 4FAH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 74 -13965 -188.71 -423.17 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -188.71 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.740

(partial model without unconserved sides chains): PDB file : Tito_4FAH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FAH-query.scw PDB file : Tito_Scwrl_4FAH.pdb: