Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFKLVDLNVERVDKEEQSIEQFPLIDGLIINKEDGENHWLIEALVQKEHLSFFEQLQNSQSEAKVFVTITKKSNRPAQLTAAVKNIVKLEESIQVLLYGQMVTRKQQGTETILES---LVKE-GYTGTKLIEAFKQKL 4ZEK Chain:A ((253-339)) ---------------------------------------------LIENFHMFTFNMNQEVFKDKKLRRALALAVNRPIMVEALWKKQASIPAGFNFPNYGETFDPKRKAMEYNVEEAKRLVKESGYDGTPI--------

General information: TITO was launched using: RESULT: Template: 4ZEK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 202 218 1.08 2.62 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 1.08 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_4ZEK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZEK-query.scw PDB file : Tito_Scwrl_4ZEK.pdb: