Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEWNKTKSIFIVAFLILDIFLGYQFFQKWQATGKEYEVIKNDVEHDMKADHITYEGLNKEATEGYRITANQKSFSKEEIEALKDQKPLMDMPSDDHKVTSLKMKFANPIALSKKDIEDDAQALVSSKIQDG----EKYKLWKV------------DKSKKEIIFFQTYEGHYIYQKTD-----NPSNMIGQVVLHLNGKNEVVSYDQTTLETFKQI-QKESLITEMDAVELLYYQN---QLKEYSTVKSCKFGYVAQYPLTSTQVLAPVWRITVEYEKKVNGEKK---TVQEYFTVNALESTILDTDQ 5COZ Chain:A ((163-357)) --------------------------------------------------------------------------------------------------------------MSAEEAEELAVKTAKELEINVCEKNELYVLDDKNTLIFPEDDTDKQNDTYVFFMFPDVYGIPYSRCPENEALTGYANQENHLVIAMDEK-GISFLDIPPLYDWVETTETGEILHPSSILSKEVDKLKKYVTSGDIEVSEISLEYMLFADKNETYDIKPVWVVYYYQNQLVTGENSYTQKMALYDVYDAYTGEEYRI--

General information: TITO was launched using: RESULT: Template: 5COZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 579 27386 47.30 163.99 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 47.30 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_5COZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5COZ-query.scw PDB file : Tito_Scwrl_5COZ.pdb: