Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence IVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK U100 Chain:A ((16-244)) IVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQ-KRFKVRVGDRNTEQ----EAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLP-RDWAEST--TQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGY--KQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARK-KYGIYTKVTAFLKWIDRSMKA--------------------

General information: TITO was launched using: RESULT: Template: U100.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1374 -78944 -57.46 -354.01 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.95 3D Compatibility (PKB) : -57.46 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.95 QMean score : 0.721

(partial model without unconserved sides chains): PDB file : Tito_U100.pdb: (Unconserved sides chains are recalculated) : Sequence: align-U100-query.scw PDB file : Tito_Scwrl_U100.pdb: