Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMTASQQQIQLARRPQGIPVHEDFRFETIPV---PEPKQGEVLVKTLYVSVDPYMRGRMQDTKSYVEPFALDKALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAV-SESALRKIDTS---LAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS
4HFM Chain:A ((3-342))EEVSNKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRMRK-----ESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITP--TLFKIHDKDVPLSYY---TGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMKLYGRIAVCGMISQYNLEQT---EGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVSR--


General information:
TITO was launched using:
RESULT:

Template: 4HFM.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1928 -188680 -97.86 -582.34
target 2D structure prediction score : 0.57
Monomeric hydrophicity matching model chain A : 0.81

3D Compatibility (PKB) : -97.86
2D Compatibility (Sec. Struct. Predict.) : 0.57
1D Compatibility (Hydrophobicity) : 0.81
QMean score : 0.520

(partial model without unconserved sides chains):
PDB file : Tito_4HFM.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-4HFM-query.scw
PDB file : Tito_Scwrl_4HFM.pdb: