Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMMDANEIISFIQNSTKSTPVKVYVKGELEGINFGESAKAFINGNTGVVFGEWSEIQTAIEENQSKIEDYVVENDRRNSAIPMLDLKNIKARIEPGAIIRDQVEIGDNAVIMMGASINIGSVIGEGTMIDMNVVLGGRATVGKNCHIGAGSVLAGVIEPPSAKPVVIEDDVVIGANAVVLEGVTVGKGAVVAAGAIVV---------------NDVEPYTVVA-GTP-----------AKKIKDIDEKTKGKTEIKQELRQL 3GOS Chain:A ((104-274)) ------------------------------------------------------------------------------------------GFRVVPPATVRKGAFIARNTVLMPSY-VNIGAFVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIEDNCFVGARSEVVEGVIVEEGSVISMGVFIGQSTRIYDRETGEVHYGRVPAGSVVVSGNLPSKDGSYSLYCAVIVKKVDA------GINELLRT-

General information: TITO was launched using: RESULT: Template: 3GOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 820 -147343 -179.69 -1067.70 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -179.69 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.685

(partial model without unconserved sides chains): PDB file : Tito_3GOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GOS-query.scw PDB file : Tito_Scwrl_3GOS.pdb: