Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIAIDLDGTLLNSKHQVSLENENALRQAQRDGIEVVVSTGRAHFDVMSIFEPLGIKTWVISANGAVIHDPEGRLYHHETIDKKRAYDILSWLESENYYYEVFTGSAIYTPQNGRELLDVELDRFRSANPEADLSVLKQAAEVQYSQSGFAYINSFQELFEADEPIDFYNILGFSFFKEKLEAGWKRYE-HAEDLTLVSSAEHNFELSSRKASKGQALKRLAKQLNIPLEETAAVGDSLNDKSMLEAAGKGVAMGNAREDIKSIADAVTLTNDEHGVAHMMKHLL 3FZQ Chain:A ((5-271)) YKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICTGRSMGTIQDDVLSLGVD-GYIAGGGNYIQY-HGELLYNQSFNQRLIKEVVCLLKKREVAFSIESQEKVFMNQKAKEI-------FETMN-QLKGTNSCINK-QHIQEK-ITYEN---NIE-EYKSQDIHKICLWSNEK-VFDEVKDILQDKME-LAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKETICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRR-

General information: TITO was launched using: RESULT: Template: 3FZQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1558 -64865 -41.63 -243.85 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -41.63 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_3FZQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FZQ-query.scw PDB file : Tito_Scwrl_3FZQ.pdb: