Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEVNG-RVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAETPEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYT-NGQTANAALIHQKLLPIMKELFKAPNPAPVKTALQLRGL-DVGSVRLPLVPLTEDERLSLSSTISEL 5F1V Chain:D ((10-275)) -------TALITPFKN-GKVDEQSYARLIKRQIENGIDAVVPVGTTGESATLTHEEHRTCIEIAVETCKGTKVKVLAGAGSNATHEAVGLAKFAKEHGADGILSVAPYYNKPTQQGLYEHYKAIAQSVDIPVLLYNVPGRTGCEISTDTIIKLFRDCENIYGV-EASGNIDKCVDLLAHEPRMMLI-SGEDAINYPILSNGGKGVISVTSNLL-PDMISALTHFALDENYKEAKKINDELYNINKILFCESNPIPIKTAMYLAGLIESLEFRLPLC-----------------

General information: TITO was launched using: RESULT: Template: 5F1V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1458 -133899 -91.84 -511.06 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -91.84 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_5F1V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F1V-query.scw PDB file : Tito_Scwrl_5F1V.pdb: