Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIGIFGGTFDPPHNGHLLMANEVLYQAGLDEIWFMPNQIPPHKQNEDYTDSFHRVEMLKLAIQSNPSFKLELVEMEREGPSYTFDTVSLLKQRYPNDQLFFIIGADMIEYLPKWYKLDELLNLIQFIGVKRPGFHVETPYPLLFADVPEFEVSSTMIRERFKSKKPTDYLIPDKVKKYVEENGLYES 1KAM Chain:A ((1-189)) SKKIGIFGGTFDPPHNGHLLMANEVLYQAGLDEIWFMPNQIPD---------SFHRVEMLKLAIQSNPSFKLELVEMEREGPSYTFDTVSLLKQRYPNDQLFFIIGADMIEYLPKWYKLDELLNLIQFIGVKRPGFHVETPYPLLFADVPEFEVSSTMIRERFKSKKPTDYLIPDKVKKYVEENGLYES

General information: TITO was launched using: RESULT: Template: 1KAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 816 -120802 -148.04 -671.12 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -148.04 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.600

(partial model without unconserved sides chains): PDB file : Tito_1KAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KAM-query.scw PDB file : Tito_Scwrl_1KAM.pdb: