Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MTHQIVTTQYGKVKGTTEN----GVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGSICPQPSDLLSLSYTELP------------RQSEDCLYVNVFAP-DTPSKNLPVMVWIHGGAFYLGAGSEPLYDGSKLAAQGE-VIVVTLNYRLGPFGFLHLSSFNEAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAA--KGLFQKAIMESGASR----TMTKEQAASTSAAFLQVLG--INEGQLDKLHTVSAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKAIAEGAASGIPLLIGTTRDEGYLFFTPDSDVH--------SQETLDAALEYLL---GKPLAEKVADLYPRS----------LESQIHMMTDLLFWRPAVAYASAQSHY-APVWMYRFDWHPKK---PPYNKAFHALELPFVFGNLDGLERMAKAEITDEVKQLSHTIQSAWITFAKTGNPST----EAVNWPAY--HEETRETLILDS---EITIENDPESEKRQKLFPSKGE------ 1MAH Chain:A ((4-543)) EDPQLLVRVR-GGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYP------GFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVE-GAVLVSMNYRVGTFGFLALPG-SREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRS--LFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCN-DTELIACLRTRPAQDLVDHEWHVLPQ-ESIFR-FSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYT-DWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFG----LPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTT--AAQQYVSLNLKPL--EVRR-GLRAQTCAFWNRFLPKLLSAT

General information: TITO was launched using: RESULT: Template: 1MAH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2771 -27806 -10.03 -59.80 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -10.03 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1MAH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1MAH-query.scw PDB file : Tito_Scwrl_1MAH.pdb: