Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAG-GIWTE----DASRMEAM-SLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAG--SRHVYELHGSIQTAACPACGARYD----LPHLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV-MHFDTLYEKLDQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTYCDSLFDMVIHQKIGEFARSLGMKK 4BVH Chain:A ((23-211)) -----------QRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELY----PGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCP-------VCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEP-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 802 -72395 -90.27 -411.34 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -90.27 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_4BVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BVH-query.scw PDB file : Tito_Scwrl_4BVH.pdb: