Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANQKKKTLPPQHQNQQPGFEYLMDPRPVFDKPKKAKKLEGKTAIITGGDSGIGRAVSVLFAKEGANVVIVYLNEHQDAEETKQYVEKEGVKCLLIAGDV---GD-EAFCNDVVGQASQVFPSIDILVNNAAEQHVQPSIEKITSHQLIRTFQTNIFSMFYLTKAVLPHLKKG--SSIINTASITAYKGNKTLIDYSATKGAIVTFTRSLSQSLVQQGIRVNAVAPGPIWTPLIPASFAAKDVEVFGSDV-PMERPGQPVEVAPSYLYLASDDSTYVTGQTIHVNGGTIVNG 2EWM Chain:B ((3-247)) ------------------------------------QRLKDKLAVITGGANGIGRAIAERFAVEGADIAIADLVPAPEAEAA---IRNLGRRVLTVKCDVSQPGDVEAFGKQVI----STFGRCDILVNNAGIYPLIP-FDELTFEQWKKTFEINVDSGFLMAKAFVPGMKRNGWGRIINLTSTTYWLKIEAYTHYISTKAANIGFTRALASDLGKDGITVNAIAPSLVRTATTEASALSAMFDVLPNMLQAIPRLQVPLDLTGAAAFLASDDASFITGQTLAVDGGMV---

General information: TITO was launched using: RESULT: Template: 2EWM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1285 -140988 -109.72 -592.38 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -109.72 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_2EWM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EWM-query.scw PDB file : Tito_Scwrl_2EWM.pdb: