Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQINIKKRMIAVHAEDASGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGYVPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAIAIER 3UWB Chain:A ((2-150)) ---SLKIKTIGDRCLRQKSEEVEF-DKKEMSELYDQMCEAMWA------------SDGIGLAAPQVGINKRVIVVDETTEEHGKYAHLMVNPKITWKSEE-KV-LFDEGCLSVP-DQNGEVLRPKSIKVTFQNKDGKYKKWKLDGLAARVVQHEIDHLEGILFVDYFN----------------

General information: TITO was launched using: RESULT: Template: 3UWB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 741 -54286 -73.26 -364.33 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -73.26 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.461

(partial model without unconserved sides chains): PDB file : Tito_3UWB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UWB-query.scw PDB file : Tito_Scwrl_3UWB.pdb: