Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPINIQDQFLNQIRKENTYVTVFLLNGFQLRGQVKGFDNFTVLLESEGKQQLIYKHAISTFAPQKNVQLELE 3QUI Chain:E ((6-70)) ----SLQDPYLNTLRKERVPVSIYLVNGIKLQGQIESFDQFVILLKNT-VSQMVYKHAISTVVPSRPVRL---

General information: TITO was launched using: RESULT: Template: 3QUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 234 -42561 -181.88 -654.78 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain E : 0.85 3D Compatibility (PKB) : -181.88 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_3QUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3QUI-query.scw PDB file : Tito_Scwrl_3QUI.pdb: