Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVEQIMKRDVITLTKTDTLETAICKLKEFHIRHLPVVDEERHVIGMITDRDMKQASPSIFEENKRSLFLTRSVDSIMKKDVVCAHPLDFVEEISAVFYEHG-IGCLPVVHH-QKLIGILTKTDLLRTFVKLTGADQPGSQIEIKVNDITKSLAEISSLCQDLQVKILSVLVYPHDDPGVKVLVFRVKTMNPLPFLQALQRNGHHVVWPSEQRDLL 2P9M Chain:A ((9-135)) -KVKDVMTKNVITAKRHEGVVEAFEKMLKYKISSLPVIDDENKVIGIVTTTDIGYNLIRDK------YTLETTIGDVMTKDVITIHEDASILEAIKKMDIS-IINQLPVVDKNNKLVGIISDGDIIRTIS--------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2P9M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 542 -66360 -122.43 -553.00 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -122.43 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_2P9M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P9M-query.scw PDB file : Tito_Scwrl_2P9M.pdb: