Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMMKYEAVIIGGGPVGFMLASELAIAGVGTCVIERLEKPVPHSKALTLHPRTLELLEMRGILERFVSKGSKIPTGHFSMLDTRLDFSGL----------DTSCPYTLL-------LPQSKTEKLLEDHARSLGTEVFRGAEALAVTQNGEAVQTIFKDR-DGSVRTITSKFAVGADGAGSTVRKQAKIEFPGTDSTVTAALGDVVLLSPPPSGVLSLCT-KEGGVMIVPLSPDRYRVVVISPYRTQTPKDVPVTEEELKADLLRICGTDFGLTDPSWMSRFGNAARQAKRYRDGRIFLAGDAAHIHFPAGGQGLNVGLQDAMNLGWKLAAAIKGSAPSWLLDSYHDERHPAAEGLLRNTEAQTKLIDFTQAGLHL-----RSMMSELLA-FPDVNRYVAGQISALDVRYEADRT------------------------------------MPPNRLNGARLPDMKLILSDGNSERLYSFLQNGTFVLLS------LRQEADDHIEVKGLRTVTASLAEPNEKLRNVH-------------TILIRPDGHVAWAVDASAPDCSEVIQKGISRWFSVTSRV 4Z2T Chain:A ((11-591)) --AETDVLIVGAGPAGAMSATLLASLGIRSLMINRWRSTSPGPRSHIINQRTMEILRDIGLEESAKSLAVPKEYMGEHVYATSLAGEEFGRIPAWASHPQAHAEHELASPSRYCDLPQLYFEPMVVSEAALRGADVRFLTEYLGHVEDQDGVTARLLDHVSGAEYEVRAKYIIGADGAHSLVAQNAGLPFEGQ-------MGGSINIEFSAD-LSSLCEHRKGDMYYMFV----AALRMIRPWNKWICVWGEITKEEAKKIIHEIIGTDEIPVEVGPISTWTINQQYAVRNTSGRVFCMGDAVHRHTPMGGLGLNTSVQDAYNLAWKLALVLKGTAAPTLLDSYDAERSPVAKQIVERAFKSLSTFPPVFEALSLPPAPTESEMAEALVRLKDASEEGAKRRAALRKAMDATIIGLGGGHGVELNQRYVSRAVFPDGTPDPGFVRDQEFFYQASTRPGAHLP-HVWLTENQRRISTLDLCGKGRFTLLTGLSGAAWKHEAEQVSQSLGIE-LKVCVIGPGQEFVDTYGEYAKISEIGESGALLVRPDMFIAFRAKDASREGLEQLNVAVKSILGR----

General information: TITO was launched using: RESULT: Template: 4Z2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2594 -254001 -97.92 -530.27 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -97.92 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_4Z2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z2T-query.scw PDB file : Tito_Scwrl_4Z2T.pdb: