Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQINIKKRMIAVHAEDASGKLYSYALFNPKIVSHSVEK-SYLTSGEGCLSVDEA-IPGYVPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAIAIER 2OKL Chain:A ((2-185)) MLTMKDVIREGDPILRNVAEEVSLPASEEDTTTLKEMIEFVINSQDPEMAEKYSLRPGIGLAAPQIGVSKKMIAVHVTDADGTLYSHALFNPKIISHSVERTYL-QGGEGCLSVD-REVPGYVPRYTRITVKATSINGEEVKLRLKGLPAIVFQHEIDHLNGVMFYDHINKENPFAAPDDSKPLER

General information: TITO was launched using: RESULT: Template: 2OKL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 922 -71319 -77.35 -391.86 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -77.35 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_2OKL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OKL-query.scw PDB file : Tito_Scwrl_2OKL.pdb: