Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISFIFAMDANRLIGKDNDLPWHLPNDLAYFKKITSGHSIIMGRKTFESI-GRPLPNRKNIVVTSAPDSEFQ-GCTVVSSLKDVLDICSGP-EECFVIGGAQLYTDLFPYADRLYMTKIHHEFEGDRHFPEFDESNWKLVSSEQG---TKDEKNPYDYEFLMYEKKNSSKAGGF 4DFR Chain:A ((1-159)) MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLDKPVIMGRHTWESIG-RPLPGRKNIILSSQP--GTDDRVTWVKSVDEAIAACGDVP-EIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFS---EFHDADAQNSHSYCFKILERR--------

General information: TITO was launched using: RESULT: Template: 4DFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 697 -28305 -40.61 -185.00 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -40.61 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.608

(partial model without unconserved sides chains): PDB file : Tito_4DFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DFR-query.scw PDB file : Tito_Scwrl_4DFR.pdb: