Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIAIDLDGTLLNSKH-QVSLENENALRQAQRDGIEVVVSTGRAHFDVMSIFEPLGIKTWVISANGAVIHDPEGRLYHHETIDKKRAYDILSWLESE--NYYYEVFTGSAIYTPQNGRELLDVELDRFRSANPEADLSVLKQAAEVQYSQSGFAYINSFQELFEADEPIDFYNILGFSFFKEKLEAGWKR--YEHAE-DLTLVSSAEHNFELSSRKASKGQALKRLAKQLNIPLEETAAVGDSLNDKSMLEAAGKGVAMGNAREDIKSIADAVTLTNDEHGVAHMMKHLL 3DAO Chain:A ((21-283)) IKLIATDIDGTLVKDGSLLIDPEYMSVIDRLIDKGIIFVVCSGRQFSSEFKLFAPIKHKLLYITDGGTVVRTP-KEILKTYPMDEDIWKGMCRMVRDELPACDYFAATPDFCFAEDGGSPI----FHLLRDSY-G----------------FEMREVDDITR----LDRNDIIKFTVFHPDK--CEELCTPVFIPAWNKKAHLAAAGKEWVDCNAKGVSKWTALSYLIDRFDLLPDEVCCFGDNLNDIEMLQNAGISYAVSNARQEVIAAAKHTCAPYWENGVLSVLKSFL

General information: TITO was launched using: RESULT: Template: 3DAO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1377 11682 8.48 45.46 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 8.48 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3DAO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DAO-query.scw PDB file : Tito_Scwrl_3DAO.pdb: