Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKLDLQPTEKLFIPFITAGDPVPEVSIELAKSLQKAGATALELGVAYSDPLADGPVIQRASKRALDQGMNIVKAIELGGEMKKNGVNIPIILFTYYNPVLQLNKEYFFALLQENHIDGLLVPDLPLEESNSLQEECKSHEVTYISLVAPTS-ESRLKTIIEQAEGFVYCVSSLGVTGVRNE-FNSSVYPFIRTVKNLSTVPVAVGFGISNREQVIKMN-EISDGVVVGSALVRKIEELKDRLISAETRNQALQEFEDYAMAFSGLYSLK 1RD5 Chain:A ((15-261)) -------KGKTAFIPYITAGDPDLATTAEALRLLDGCGADVIELGVPCSDPYIDGPIIQASVARALASGTTMDAVLEMLREVTP-ELSCPVVLLSYYKPIMF----RSLAKMKEAGVHGLIVPDLPYVAAHSLWSEAKNNNLELVLLTTPAIPEDRMKEITKASEGFVYLVSVNGVTGPR-ANVNPRVESLIQEVKKVTNKPVAVGFGISKPEHVKQIAQWGADGVIIGSAMVRQLGEAASP-------KQGLRRLEEYARGMKNAL---

General information: TITO was launched using: RESULT: Template: 1RD5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1346 -196043 -145.65 -803.45 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -145.65 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_1RD5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RD5-query.scw PDB file : Tito_Scwrl_1RD5.pdb: