Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITMENIVRDGHPALRETAEPVELPPTDAEKQQLADMIEFVKNSQNPELAEKYKLRPGVGLAAPQINIKKRMIAVHAEDASGKLYSYALFNPKIVSHSVEKSYLTSGEGCLSVDEAIPGYVPRYARIRVKGTTLEGENIDIRLKGFPAIVFQHEIDHLNGVMFYDHIDKENPFKEPENAIAIER 4WXK Chain:A ((3-159)) ---ALNVLIYPDDHLKVVCEPVTE-VNDAIRKIVDDMFDTMYQ------------EKGIGLAAPQVDILQRIITIDVEGD--KQNQFVLINPEILASEGE--T-GIEEGCLSIP-GFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVDYLSPLKRQRIKEKL-----

General information: TITO was launched using: RESULT: Template: 4WXK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 804 -79292 -98.62 -505.04 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -98.62 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.389

(partial model without unconserved sides chains): PDB file : Tito_4WXK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WXK-query.scw PDB file : Tito_Scwrl_4WXK.pdb: