Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASIAVCPGSFDPVTYGHLDIIKRGAHIFEQVYVCVLNNSSKKPLFSVEERCELLREVTKDIPNITVETSQGLLIDYAKRKNAKAILRGLRAVSDFEYEMQGTSVNRVLDESIETFFMMTNNQYSFLSSSIVKEVARYNGSVSEFVPPEVELALQQKFRQG 4RUK Chain:A ((2-158)) --NRVLYPGTFDPITKGHGDLIERASRLFDHVIIAVAASPKKNPLFSLEQRVALAQEVTKHLPNVEVVGFSTLLAHFVKEQKANVFLRGLRAVSDFEYEFQLANMNRQLAPDVESMFLTPSEKYSFISSTLVREIAALGGDISKFVHPAVADALAERFK--

General information: TITO was launched using: RESULT: Template: 4RUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 753 -103454 -137.39 -658.94 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -137.39 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_4RUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RUK-query.scw PDB file : Tito_Scwrl_4RUK.pdb: