Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIIQGNLVGTGLKIGIVVGRFNDFITSKLLSGAEDALLRHGVDTNDIDVAWVPGAFEIPFAAKKMAETKKYDAIITLGTVIRGATTHYDYVCNEAAKGIAQAANTTGVPVIFGIVTTENIEQAIERAGTKAGNKGVDCAVSAIEMANLNRSFE 1NQX Chain:D ((1-153)) MQIYEGKLTAEGLRFGIVASRFNHALVDRLVEGAIDCIVRHGGREEDITLVRVPGSWEIPVAAGELARKEDIDAVIAIGVLIRGATPHFDYIASEVSKGLANLSLELRKPITFGVITADTLEQAIERAGTKHGNKGWEAALSAIEMANLFKSL-

General information: TITO was launched using: RESULT: Template: 1NQX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 815 -116085 -142.43 -758.72 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.89 3D Compatibility (PKB) : -142.43 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_1NQX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NQX-query.scw PDB file : Tito_Scwrl_1NQX.pdb: