Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQNKCALVTGSSRGVGKAAAIRLAENGYNIVINYARSKKAALETAEEIE-KLGVKVLVVKANVGQPAKIKEMFQQIDETFGRLDVFVNNAASGVL---RPVMELEETHWDWTMNINAKALLFCAQEAAKLMEKNGGGHIVSISSLGSIRYLENYTTVGVSKAALEALTRYLAVELSPKQIIVNAVSGGAIDTDALKHFPNREDLLEDARQNTPAGRMVEIKDMVDTVEFLVSSKADMIRGQTIIVDGGRSLLV 2CFC Chain:A ((1-249)) --MSRVAIVTGASSGNGLAIATRFLARGDRVAALD-LSAETLEETARTHWHAYADKVLRVRADVADEGDVNAAIAATMEQFGAIDVLVNNAGITGNSEAGVLHTTPVEQFDKVMAVNVRGIFLGCRAVLPHMLLQGAGVIVNIASVASLVAFPGRSAYTTSKGAVLQLTKSVAVDYAGSGIRCNAVCPGMIETPMTQWRLDQPELRDQVLARIPQKEIGTAAQVADAVMFLAGEDATYVNGAALVMDGAYTA--

General information: TITO was launched using: RESULT: Template: 2CFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1389 -146747 -105.65 -598.97 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -105.65 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.645

(partial model without unconserved sides chains): PDB file : Tito_2CFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CFC-query.scw PDB file : Tito_Scwrl_2CFC.pdb: