Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGTSSRSTKLVHGGLRYLKQFEVKMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFTTSIGLRVYDFLAGVKKSERRSMLSAKETLQKE---PLVKKDGLKGGGYYVEYRTDDARLTIEVMKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLREKDHSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIP--REGKT--YVGTTDTVYKEALEHPRMTTEDRDYVIKSINYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKDEIWTSDSGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGEKDFGPCKTKNMPISGGHVGGSKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHILAEAEYSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTENLNKLLHDAVVPLEQ 5EZ7 Chain:A ((2-392)) ----------------MPQALSTDILIVGGGIAGLWLNARLRRAGYATVLVESASLGGGQSVKSQGIIHGGT--------------GASEAIAD-------------MPRRWRACLGS-DGELDLRGVRLLS-----EAHYLWSPVEQAKGEDLPPALRDKGFKGKAYRLTEIVFDVPDLIRRLAELAG---DSLLAGERIEPLREGRELAGLCVDG------REIRAQRVVLSAGAGNEALLRELGLEQPAMQRRPLHMVMVKAATLKPLYAHCLGAGPKPRITVTTHPTRDGQSVWYLGG-DIAETDGVARD--EAAQIAEARRELAKLLPWIDLG----QAQWATLRVDRAEPAQ--SNLLRPDNAFLAEQGRLLVGW---PTKLALAPDFADRVCARLEEDGIRPSEHAALPQLPRPP--LAEPAWEVAFA-------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EZ7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2045 -143004 -69.93 -401.70 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -69.93 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.394

(partial model without unconserved sides chains): PDB file : Tito_5EZ7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZ7-query.scw PDB file : Tito_Scwrl_5EZ7.pdb: