Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEKKERIIKTSIRLFA-KKGFAATTIQEIASECGISKGAFYLHFKSKEALLLSACEYYIGMSMNKMKNIEEDLAGKPPKEVLKKQIGAQFEDFRDHKDFIVLLLTENIIPENQEIKQYFYKVTMETDKLYRNALLVSY---GEGIERYVADLSIMARGIVHSYMNVMVFNGELNIDAEEISAFIIERLDDLVQGLSRSALNPIVSKDIFNPMPAGKDQLLEDIQKVKENSTLPEDITVSLDVIEEELTQDKPRKPIIKGMLSNLAGTNDKEVERLRALILSLSQFDHKKSSL 4GFK Chain:A ((8-176)) --NRREEILQALAEMLESN-----ITTAKLAKQVGVSEAALYRHFPSKTRMFEGLIEFIEESLMSRINRIFD--EEKDTLNRIRLVMQLLLAFAERNPGLTRILSGHALMFENERLRDRINQLFERIETSLRQILRERKLREGKSFPVDENILAAQLLGQVEGSLNRFVRSDF------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GFK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 555 -88642 -159.72 -554.01 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -159.72 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_4GFK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GFK-query.scw PDB file : Tito_Scwrl_4GFK.pdb: