Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAISGGGTGGHIYPALAFIKEVQRRHPNVEFLYIGTENGLEKKIVERENIPFRSIEITGFKRKL-----------SFENV-KTVMRFLKGVKKSKSYLAEFKPDAVIGTGGYVCGPVVYAAAKMGIPTIVHEQNSLPG-----------------------I---TNKFLSK-------------YVNKVAICFEEAKSHFP---SEKVVFTGNPRASEVVSIKTGRSLAEFGLSEDKKTVLIFGGSRG--AAPINRAVIDMQDVLKTRDYQVLYITGEVHYEKVMNELKSKGAADNMVTKPFLHQMPEYLKAIDVIVARAGATTIAEITALGIPSVLIPSPYVTANHQEVNARSLGQHDAAIVLKETELSGEKLIEALDRIVLNEQTLKEMSERTKSLGVPDAAARLYSVLEELKK 3RSC Chain:A ((21-411)) AHLLIVNVASHGLILPTLTVVTELV-RR-GHRVSYVTAGGF-AEPVRAA-GATVV--PYQSEIIDADAAEVFGSDDLGVRPHLMYLRENVSVLRATAEALDGDVPDLVLYDDFPF-IAGQLLAARWRRPAVRLSAAFASNEHYSFSQDMVTLAGTIDPLDLPVFRDTLRDLLAEHGLSRSVVDCWNHVEQLNLVFVPKAFQIAGDTFDDRFVFVGPCFDDRRFLGE------WTRPADDLPVVLVSLGTTFNDRPGFFRDCARAFDGQ---PWHVVMTLGGQVDPAA--L---GDLPPNVEAHRWVPHV-KVLEQATVCVTHGGMGTLMEALYWGRPLVVVPQS----FDVQPMARRVDQLGLGAVLPGEKADGDTLLAAVGAVAADPALLARVEAMRGHVRRAGGAARAADAVEAY--

General information: TITO was launched using: RESULT: Template: 3RSC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1976 -248017 -125.51 -740.35 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -125.51 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_3RSC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RSC-query.scw PDB file : Tito_Scwrl_3RSC.pdb: