Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANQKKKTLPPQHQNQQPGFEYLMDPRPVFDKPKKAKKLEGKTAIITGGDSGIGRAVSVLFAKEGANVVIVYLNEHQ-----------DAEETKQYVEKEGVKCLLIAGDVGDEAFCNDVVGQASQVFPSIDILVNNAAEQHVQPSIEKITSHQLIRTFQTNIFSMFYLTKAVLPHLKK---GSSIINTASITAYK----GNKTLIDYSATKGAIVTFTRSLSQSLVQQGIRVNAVAPGPIWTPLIPASFAAK-----DVEV---FGSDVPMERPGQPVEVAPSYLYLASDDSTYVTGQTIHVNGGTIVNG 4RGB Chain:A ((21-292)) ------------------------------------GSLDGRVVFITGAARGQGRSHAVMCAEQGANIVGVDICEDIDIVPYKLGTYEELEETARLVEKTGQEMLFRKADVRDKAVLQEVFDAGVEQFGHIDTVIANAGVVLTN-PDERDASEALRLGLDIMLIGVWNTFQVAIPHMKERGQGGNLIATSSMIALLDLTDGRGGTDAYLTSKLAITGLVRSYALMLAADRIRVNGVAPTNCSTPMITENPALFKVIEENPHLVNAMSTALPDFPMIEPRDVSNAILFLISDAGRSFTGSVLKVDAGMDV--

General information: TITO was launched using: RESULT: Template: 4RGB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1426 -146875 -103.00 -597.05 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -103.00 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_4RGB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RGB-query.scw PDB file : Tito_Scwrl_4RGB.pdb: