Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKEFAVIGLGRFGGSICKALSEEGVEVMAMDIDEDKVNEYAKIASHAVIGDSTDESVLKNLGLRNFDHVIVAIGENIQASILTTLILKELGVHTITVKAQNDYHEKVLSKIGADHIVHPERDMAKRIAHNIVSNNVLDYLELSEEHSLVEIVANSRLAGNTLLDLDIRAKYGINIVAIKRGKEVIVSPLATEVIHQEDILIVIGSVTDISRFEKRVLHTK 2HMT Chain:A ((6-143)) -NKQFAVIGLGRFGGSIVKELHRMGHEVLAVDINEEKVNAYASYATHAVIANATEENELLSLGIRNFEYVIVAIGANIQASTLTTLLLKELDIPNIWVKAQNYYHHKVLEKIGADRIIHPEKDMGVKIAQSLSDENVLN----------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2HMT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 647 -106278 -164.26 -770.13 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -164.26 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.688

(partial model without unconserved sides chains): PDB file : Tito_2HMT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HMT-query.scw PDB file : Tito_Scwrl_2HMT.pdb: