Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGEIRLIPYVTNEQIMDVNELPEGIKVIKAPEMWAKGVKGKNIKVAVLDTGCDTSHPDLKNQIIGGKNFTDDDGGKEDAISDYNGHGTHVAGTIAANDSNGGIAGVAPEASLLIVKVLGGENGSGQYEWIINGINYAVEQKVDIISMSLGGPSDVPELKEAVKNAVKNGVLVVCAAGNEGDGDERTEELSYPAAYNEVIAVGSVSVARELSEFSNANKEIDLVAPGENILSTLPNKKYGKLTGTSMAAPHVSGALALIKSYEEESFQRKLSESEVFAQLIRRTLPLDIAK-TLAGNGFLYLTAPDELAEKAEQSHLLTL 1TO2 Chain:E ((3-276)) --------------------SVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGG--ASMVPSE---TNPFQDNNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGA-DGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGS-SSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHP-----NWTNTQVR-SSLENTT-TKLGDSFYYGKGLINVQAAAQH------------

General information: TITO was launched using: RESULT: Template: 1TO2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1953 -168106 -86.08 -615.77 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain E : 0.80 3D Compatibility (PKB) : -86.08 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_1TO2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TO2-query.scw PDB file : Tito_Scwrl_1TO2.pdb: