Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNINVDVKQNENDIQVNIAGEIDVYSAPVLREKLVPLAEQGADLRIC--LKDVSYMDSTGLGVFVGTFKMVKKQGGSLKLENLSERLIRLFDITGLKDIIDISAKSEGGVQ 1H4Y Chain:A ((15-95)) ---------------IRLFGELDHHAVEQIRAKISTAIFQGAVTTIIWNFERLSFMDSSGVGLVLGRMRELEAVAGRTILLNPSPTVRKVFQFSGL---------------

General information: TITO was launched using: RESULT: Template: 1H4Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 260 -54817 -210.83 -693.88 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -210.83 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_1H4Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1H4Y-query.scw PDB file : Tito_Scwrl_1H4Y.pdb: