Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQIGDNIVYPMHGAGIIEAIEEKEFLEEKQQYYVIRMSISNMTVMIPTSKILSSNIRPVTDILALKHIMHIFQHGESDRLLPWKQRYKINTDKIKTGEIQEGAEVVRDLMRMKKEKALNASEKKMLDNAYEFLISELEVIKGITEKQIKSFG 4XAX Chain:B ((2-152)) -FRPGDKVVLPPYGVGVVAGIAQRSVSGVSRAYYQVDFPGSRSKAYVPVEAPHSVGLRKALAPEEVPVILDLLKNGRMPLPKQWAARHRKTSEILADGNPYRIAQMAGQLRAWEVERGLPDLDRQALRRAIHLLAEEVAQSLEITVQEAKRL-

General information: TITO was launched using: RESULT: Template: 4XAX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 590 -64195 -108.80 -425.13 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -108.80 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_4XAX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XAX-query.scw PDB file : Tito_Scwrl_4XAX.pdb: