Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEQKLCQAINLFVEVLLEGTEFVHREINQDVFKHISREQADLLKILKVKGPTSPGSLAMYQNVHKSAISNRLKKLLEKGLVQWDDCPEKSDRRSKLINITPSGEHILEELDSAIFNALKPLIDDIDEEHLHSIIEIFTILKSKFKGGDSAE 4EJT Chain:A ((36-149)) -----------------------------------ISAEQSHVLNMLSIEALT-VGQITEKQGVNKAAVSRRVKKLLNAELVKLEK---------KIIKLSNKGKKYIKERKAIMSHIASDMTSDFDSKEIEKVRQVLEIIDYRIQSYTS--

General information: TITO was launched using: RESULT: Template: 4EJT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 246 -29854 -121.36 -284.32 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -121.36 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4EJT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EJT-query.scw PDB file : Tito_Scwrl_4EJT.pdb: