Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTENFWRELPRPFFVLAPMEDVTDVVFRHVVSEAGRPDVF---------------FTEFTNSESYCHPDGKDSVRGRLTFTEDEQPIVAHIWGDKPENFRKMSIG-MAELGFKGLDINMGCPVPNVAGNGKGSGLICRPAVAAELIQA-AKAGGLPVSVKTRLGYTDVDEWREWLTHILKQDIANLSIHLRTRAEMSKVDAHWELIPEIKKLRDEVAPDTL---LTINGDIPDRQTGLKLAEQY----GVDGIMIGRGIFTNPFAFEKEPKEHSSKELLDLLRLHLDLHDEYSKEEARPYKPLPRFFKIYLRGFRGASELRNQCMNTKSTDEVRALLDDFERKYLDGIE 4XP7 Chain:A ((20-261)) -------------LILAPMVRVGTLPMRLLALDYGADIVYCEELIDLKMIQCKRVVNEVLSTVDFVAPDDRVVFR---TCEREQNRVVFQMGTSDAE--RALAVARLVENDVAGIDVNMGCPKQ---------ALLSDPDKIEKILSTLVKGTRRPVTCKIRI-LPSLEDTLSLVKRIERTGIAAIAVHGRKREERPQHPVSCEVIKAI--------ADTLSIPVIANGGSHDHIQQYSDIEDFRQATAASSVMVARAAMWNPSIFLKE--------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4XP7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1057 -138054 -130.61 -660.55 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -130.61 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_4XP7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XP7-query.scw PDB file : Tito_Scwrl_4XP7.pdb: