Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVPKTMLLSTAAGLLLSLTATSVSAHYVNEEHHFKVTAHTETDPVASGDDAADDPAIWVHEKHPEKSKLITTNKKSGLVVYDLD-GKQLHSYEF-GKLNNVDLRYDFPLNGEKIDIAAASNRSEGKNTIEVYAIDGDKGKLKSITDPNHPISTNISEVYGFSLYHSQKTGAFYALVTGKQG---EFEQYEIVDGGKGYVTGKKVREFKLNSQTEGLVADDEYGNLYIAEE--DEAIWKFNAEPGGGSKGQVVDRATGDHLTADIEGLTIYYAPNGKGYLMASSQGNNSYAMYERQGKNRYVANFEITDGEKIDGTSDTDGIDVLGFGLGPKYPYGIFVAQDGENIDNGQAVNQNFKIVSWEQIAQHLGEMPDLHKQVNPRKLKDRSDG 3U4Y Chain:A ((10-207)) ------------------------------------------------------------------NFGIVVEQHLRRISFFSTDTLEILNQITLGYDFVDTAITSD------C-SNVVVTSDFC--QTLVQIETQLEPPKV--V-A----IQEGQSSMADVDI--TP-DD-QFAVTVTGLNHPFNMQSYSFLK-------NKFISTIPIPYDAVGIAISPNGNGLILIDRSSANTVRRFKIDADGVLFDTGQE-FISG--GTRPFNITFT--PDG-NFAFVANLIGNSIGILETQN--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3U4Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1047 37487 35.80 196.26 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 35.80 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_3U4Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3U4Y-query.scw PDB file : Tito_Scwrl_3U4Y.pdb: