Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNRIIYVVSDSVGETAELVVKAALSQFNGSADDTHVRRIPYVEDIGTINEVISLAKADGGIICFTLVVPEIREYLIAEAEKANVLYYDIIGPLIDKMETAYGLTAKYEPGRVRQLDEDYFKKVEAIEFAVKYDDGRDPRGILKADIVLIGVSRTSKTPLSQYLAH-KRLKVANVPI----------VPEV------------------------DPPEELFNVDPKKCIGLKISPDKLNHIRKERLKSLGLNDKAIYANINRIKEEL-EY-FEKIVDR-IGCQVVDVSNKAVEETANIIHHLKTKNI 4CVN Chain:A ((14-165)) --------------------------------------------------------------------------------------------------------------------------------------------------IAITGTPGVGKTTIAKLLAEKLGYEYVNLRDFALEKGCGREVDGEVEVEIDELAYFVEKELKDRNVVLDGHLSH--LMPVDLVVVLRAHPRIIGERLRERGYSKE----K-IG--ENVEAELVDAILIEAIDEHENVIEVDTTNKTPEEIVEEIIGLIKSG-

General information: TITO was launched using: RESULT: Template: 4CVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 457 24891 54.46 218.34 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 54.46 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_4CVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CVN-query.scw PDB file : Tito_Scwrl_4CVN.pdb: