Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEYTYLGRTGLRVSRLCLGT-MNFGVDTDEKTAFRIMDEALDNGIQFFDTANIYGWGKNAGLTESIIGKWFAQGGQRREKVVLATKVYEPISDPNDGPNDMRGLSLYKIRRHLEGSLKRLQTDHIELYQMHHIDRRTPWDEIWEAFETQVRSGKVDYIGSSNFAGWHLVKAQAEAEKRRFMGLVTEQHKYSLLER-TAEMEVLPAARDLGLGVVAWSPLAGGLLGGK--------ALKSNAGTRTAKRADLIEKHRLQLEKFSDL---CKELGEKEANVALAWVLANPVLTAPIIGPRTVEQLRDTIKAVEI--SLDKEILRMLNDIFPGPGGETPEAYAW 2R9R Chain:A ((5-321)) --YRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEHLMTLAYDNGINLFDTAEVYAAGK----AEVVLGNIIKKKGWRRSSLVITTKIFW-----GGKAETERGLSRKHIIEGLKASLERLQLEYVDVVFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQAEYHMFQREKVEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQAIAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENIGAIQVLPKLSSSIVHEIDSIL-------------

General information: TITO was launched using: RESULT: Template: 2R9R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1701 -12280 -7.22 -40.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -7.22 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_2R9R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R9R-query.scw PDB file : Tito_Scwrl_2R9R.pdb: