Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEARKRMVKRAVQEIKDGMNVNLGIGMPTLVANEIPDGVHVMLQSENGLLGIGPYPLEGTEDADLINAGKETITEVTGASYFDSAESFAMIRGGHIDLAILGGMEVSEQGDLANWMIPGKMVKGMGGAMDLVNGAK-RIVVIMEHVNKHGESKVKKTCSLPLTGQKVVHRLITDLAVFDFVNGR-MTLTELQDGVTIEEVYEKTEADFAVSQSVLNS 1M3E Chain:A ((263-474)) ----RERIIKRAALEFEDGMYANLGIGIPLLASNFISPNMTVHLQSENGILGLGPYPLQNEVDADLINAGKETVTVLPGASYFSSDESFAMIRGGHVNLTMLGAMQVSKYGDLANWMIPGKLVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDRKKGLTLIELWEGLTVDDIKKSTGCDFAVSPKLI--

General information: TITO was launched using: RESULT: Template: 1M3E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1074 -55390 -51.57 -263.76 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -51.57 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_1M3E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M3E-query.scw PDB file : Tito_Scwrl_1M3E.pdb: