Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIQLDQIGRMKQGKTILKNISWQIAKGDKWILYGLNGAGKTTLLNILNAYEPATTGGVNLFGKMPGKVGYSAETVRQHIGFVSHSLLEKFQEGERV--IDVVISGAFKSIGVYQDIDDEVRNEAHHLLKLVGISAKAQQYIGYLSTGEKQRVMIARALMGQPQVLILDEPAAGLDFIARESLLNILDSLSD-SYPTLAMIYVTH---FIEEITGNFTKILLLKDGESVQQGLIDDILT--SENMSRFFQKNVAVQRWNNRFSMAMLE 4U00 Chain:A ((19-241)) ----------------VLKGIHLEVAPGEKLVIIGPSGSGKSTLIRTINRLEDFQEGEVVVDG-LSVKDDRALREIRREVGMV-------FQQFNLFPHMTVLENVTLAPMRVRRWPREKAEKKALELLERVGILDQARKYPAQLSGGQQQRVAIARALAMEPKIMLFDEPTSALD---PEMVGEVLDVMRDLAQGGMTMVVVTHEMGFAREVA---DRVVFMDGGQIVEEGRPEEIFTRPKEERTRSFLQRV---------------

General information: TITO was launched using: RESULT: Template: 4U00.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -120412 for 1703 contacts (-70.7/contact) + 2D Compatibility (PS) -23311 + (NN) -12065 + (LL) 3924 1D Compatibility (HY) -16400 + (ID) 3450 Total energy: -171714.0 ( -100.83 by residue) QMean score : 0.413

(partial model without unconserved sides chains): PDB file : Tito_4U00.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U00-query.scw PDB file : Tito_Scwrl_4U00.pdb: